Branches of Napoli, Sassari and Catania


Instruments available in Naples (Pozzuoli)

LC-MS/MS: HPLC Alliance 2695 (Waters), with PDA 2996 UV detector (Waters) / QToF micro analyser (Waters) and ESI and APCI sources;

LC-MS/MS: UPLC Acquity (Waters), with an API3200 triple quadrupole analyser (ABI SCIEX) and with con ESI and APCI sources;

LC-HRMS/MS: UHPLC Infinity 1290 (Agilent), with Q-Exactive analyser (quadrupole-orbitrap hybrid, Thermo) with ESI source (140 000 FWHM resolution at m/z 200).

LC-APCI-MS (Shimadzu Corporation, Kyoto, Japan) with HPLC (LC-10ADVP) coupled to a single quadrupole MS (LCMS-2020, Shimadzu) through APCI interface for the analysis of small MW molecules with high sensitivity

LC-MS-IT-TOF (Shimadzu Corporation, Kyoto, Japan), in which APCI ionization is coupled to ion trap (IT) ion concentration and time-of-flight analysis, thereby ensuring high mass resolution (10,000 at 1000 m/z) and sensitivity

Offered Services:

-High accuracy mass determination of small organic molecole by means of ESI/MS/MS

-Lipid profiling by UHPLC-MS/MS on high resolution-accurate mass Q-Exactive platform with the support of LipidSearch Software for data processing (A. Cutignano et al. Algal Res., 2016, 17, 348-358)

-Quantitative analysis of sphingosine-1-phosphate in biological matrices by means of UPLC-MRM (Cutignano et al., POLM 2010, 93, 25-29)

-Quantitative analysis of glycerophosphoinositols (GroPIns) in biological matrices by means of UPLC-MRM (L. Grauso, S. Mariggiò, D. Corda, A. Fontana, A. Cutignano “An improved UPLC-MS/MS platform for quantitative analysis of glycerophosphoinositol in mammalian cells”, PlosOne, 2015, 10(4): e0123198. doi:10.1371/journal.pone.0123198)

-Profiling of endocannabinoids and endocannabinoid-related lipids in biological matrices (Piscitelli et al., Nutr Metab (Lond) 2011, 13, 8-51)

-Analysis of phospholipids, diacylglycerols and triacylglycerols (Piscitelli et al., NutrMetab (Lond) 2011, 13, 8-51)

-Analysis of prostaglandins in biological matrices (Silvestri et al., J Biol Chem. 2013, 288, 23307-23321)

- Analysis of endocannabinoids oxidation metabolites in biological matrices (Gatta et al., PLoSOne. 2012, 7, e31111)

-Development of new methods for the extraction, identification and quantification of new bioactive lipids in biological matrices (Piscitelli, 2015 In: Di Marzo &Wang (Eds.), Academic Press, London, 137-149; Freestone et al., Neuropharmacology. 2014, 79, 467-75)

Personnel in charge of services (Naples)

Adele Cutignano (phone: +39-081-8675313; email: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

Personnel in charge of the instruments  (Naples)

Adele Cutignano & Fabiana Piscitelli (phone: +39-081-8675193; email: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

 


Instruments available in Sassari

LC-HRMS/MS: HPLC 1200 Series (Agilent) with Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermoscientific)

LC-MS: HPLC 1100 Series - MSD SL (Agilent)

GC- MS/MS: Polaris Q Ion Trap (Thermoscientific)

GC-MS: 5977 E (Agilent) 

Offered Services:

 1) Validation of analytical methods using LC-MS e GS-MS for the identification and quantification of bioactive molecole in complex matrices

  • Pharmacokinetic studies on biological matrices (Rozzo et al., Molecular Cancer Volume 12, Issue 1, 2013, Article number 37)
  • Measurement of mycotoxins (Deoxyvalenol, Toxins T2 e HT2, Patulin, Zearalenoneetc) (Pani et al., Journal of Agricultural and Food Chemistry 2014, 62, 4969-4978)
  • Measurement of peptides (GE- B5, Bradykinin, Angiotensin II etc) in CSF and plasma (Fanciulli et al., Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2007, 852, 485-490)
  • Measurement of “synthetic drugs” in urine and plasma (Nieddu et al., Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008, 867, 126-130)

 2) Phytochemical and nutraceutic studies on endemic plants, fruit and vegetable matrices:

  • Isolation, and purification of bioactive substances (Foddai et al., Natural Product Communications. 2014,  9, 181-184)
  • Quantitative analysis of secondary metabolites (Barberis et al., Analytical Chemistry. 2014, 86, 8727-8734)
  • Quantitative and qualitative analysis of essential oils from endemic plants typical of the mediterranean area (Usai et al., Journal Of Essential Oil Research, 2010, 22, 373-380)

 3) Controls on products of organic synthesis:

  • Development of analytical methods for the chromatographic separation and characterization of complex misture (Fadda et al., Journal of Food Composition and Analysis. 2014, 35, 112–119)
  • Structure determination of organic molecules (Azzena et al., Journal of Organometallic Chemistry. 2007,  692, 3892-3900)
  • Exact mass determination
  • Quantitative and qualitative analysis of impurities

Personnel in charge of services  (Sassari)

Emanuela Azara (phone: +39- 079-2841215/235; email: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

Personnel in charge of the instruments

Emanuela Azara e Barbara Sechi (phone: +39-079-2841208 email: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

 


Instruments available in Catania

GC-MS-FID HP 5971

Offered services:

- Analysis of volatile compounds, pure or in mixtures;

- Analysis of food-derived matrices following chemical derivatization;

- Analysis of the aromatic component of food-derived matrices by means of SPME e Static HS;

- Analysis of volatile chiral compounds. 

Personnel in charge of the instruments (Catania)

Edoardo Napoli (phone: +39-095-73383: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

Tonia Strano (phone: +39- 095-7338351; email: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.)

 


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