Azara Emanuela Gigliola

Azara Emanuela Gigliola

    • Technicians
  • 30 Ottobre 2014
  • 1173

PERSONAL DETAILS


Profile:  Collaboratore Tecnico (CTER)

Office location: Sassari

E-mail address:  Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.

Alternative e-mail address: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.

Work phone #:  +39 0792841215

Lab phone #: +39 0792841235

Biography:  Born the 24nd of october 1969 in Sassari (Italy). Graduated in Toxicology, (110/110 e Lode) University of Sassari 

1989-1991 Fellowship from the National Research Council. Istituto Applicazioni Tecniche Chimiche Avanzate ai Problemi Agrobiologici Sassari. (Research Project: Molecular design of polysostituted Resorcinol).

1991-1993 Scholarship funded by the Research Consortium FORMEZ at CO.RI.SA Sardegna Sassari (Research Project "Characterization of marine ecosystems, coastal lagoons and assessment of environmental quality." 1994-2001 Research contract at the Istituto Zooprofilattico Sperimentale della Sardegna Dip. Igiene degli Allevamenti e Produzioni Zootecniche Sassari (Research projects: (a) "Validation of LC/MS method for Avermectins in Milk", (b) "Analysis of pesticide residues in matrices plants" (c) ”Hormones and metabolites”, (d) "Environmental Biomonitoring”). 

2002 -2014 Achieved permanent position as technician at the Biomolecular Chemistry Institute at the National Research Council


SCIENTIFIC ACTIVITIES


Interests

Biosample preparation methods, such as LLE (liquid-liquid extraction), SPE (solid phase extraction). Basic analytical methods: Chemical analysis; Instrumental analysis, such as HPLC, HPCE, LC/MS, GC, GC/MS, UV, etc. A strong background in chromatographic method development of various biological compounds. As a technical expert regularly I use specialized analytical techniques for metabolomics, phytochemistry structural characterization, impurities identification and trouble shooting the related technical issues. Identification of peptides and small protein by LC-MS-MS. Metabolism system in vitro with rat hepatic microsomes. Drug metabolism in rat liver in situ. Drug metabolism though whole experimental animals. Rapid PK study in drug discovery and drug metabolism research.

Major publications

  1. Barberis, A. , Spissu, Y., Bazzu, G., Fadda, A., Azara, E., Sanna, D., Schirra, , Serra, P.A. Development and characterization of an ascorbate oxidase-based sensor-biosensor system for telemetric detection of AA and antioxidant capacity in fresh orange juice. Analytical Chemistry Volume 86, Issue 17, 2 September 2014, Pages 8727-8734 
  2. Barberis, A., Spissu, Y., Fadda, A., Azara, E., Bazzu, G., Marceddu, S., Angioni, A., Sanna, D., Schirra, M., Serra, P.A. Simultaneous amperometric detection of ascorbic acid and antioxidant capacity in orange, blueberry and kiwi juice, by a telemetric system coupled with a fullerene- or nanotubes-modified ascorbate subtractive biosensor. Biosensors and Bioelectronics 2014
  3. Pani, G., Scherm, B., Azara, E., Balmas, V., Jahanshiri, Z., Carta, P., Fabbri, D., Dettori, M.A., Fadda, A., Dessì, A., Dallocchio, R., Migheli, Q., Delogu, G. Natural and natural-like phenolic inhibitors of type B trichothecene in vitro production by the wheat (Triticum sp.) pathogen Fusarium culmorum. Journal of Agricultural and Food Chemistry Volume 62, Issue 22, 4 June 2014, Pages 4969-4978
  4. Foddai, M., Kasabri, V., Petretto, G.L., Azara, E., Sias, A., Afifi, F.U., Delogu, , Chessa, M., Pintore, G. In vitro inhibitory effects of Limonium contortirameum and L. virgatum extracts from Sardinia n α-amylase, α-glucosidase and pancreatic lipase. Natural Product Communications Volume 9, Issue 2, 2014, Pages 181-184 
  5. Fadda, A., Serra, M., Molinu MG., Azara, E., Barberis, A., Sanna, D. Reaction time and DPPH concentration influence antioxidant activity and kinetic parameters of bioactive molecules and plant extracts in the reaction with the DPPH radical. Journal of Food Composition and Analysis Volume 35, Issue 2, September 2014, Pages 112–119
  6. Foddai, M., Kasabri, V., Afifi, F.U., Azara, E.,Petretto, G.L., Pintore, G. In Vitro inhibitory effects of Sardinian Pistacia Lentiscus L. and Pistacia Terebinthus L. on metabolic enzymes: pancreatic lipase, α-amylase and α-glucosidase. Starch - Starke. 08/2014
  7. Rozzo, C., Fanciulli, M., Fraumene, C., Corrias, A. , Cubeddu, T., Sassu, I.,Cossu, S., Nieddu, V., Galleri, G., Azara, E., Dettori, M.A., Fabbri, D. ,Palmieri, G., Pisano, M. Molecular changes induced by the curcumin analogueD6 in human melanoma cells. Molecular Cancer Volume 12, Issue 1, 4 May2013, Article number 37
  8. Scherm, B., Orrù, M., Balmas, V., Spanu, F., Azara, E.,Delogu, G., Hammond, M., Keller, N.P., Migheli, Q. Altered trichothecene biosynthesis in TRI6-silenced transformants of Fusarium culmorum influences the severity of crown and foot rot on durum wheat seedlings. Molecular Plant Pathology Volume 12, Issue 8, October 2011, Pages 759-771
  9. Nieddu, M., Boatto, G., Pirisi, M.A., Azara, E., Marchetti, M. LC-MS analysis of trimethoxyamphetamine designer drugs (TMA series) from urine samples. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Volume 867, Issue 1, 1 May 2008, Pages 126-130
  10. Fanciulli, G., Azara, E., Wood, T.D., Delitala, G., Marchetti, M. Liquid chromatography-mass spectrometry assay for quantification of Gluten Exorphin B5 in cerebrospinal fluid. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Volume 852, Issue 1-2, 1 June 2007, Pages 485-490

Keyword

LC/MS, HPLC, GC/MS, ANALITYCAL CHEMISTRY, PHYTOCHEMISTRY


OTHER