Nicolaus Barbara

Nicolaus Barbara

    • Researchers
  • 30 Ottobre 2014
  • 1219

PERSONAL DETAILS


Profile: Dirigente di ricerca

Office location: Napoli

E-mail address: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.

Work phone #: 081 8675245

Lab phone #: 0818675190

Biography:

La Dott.ssa Barbara Nicolaus e' nata a Napoli il 9/10/1953 e conseguiva la laurea in Scienze Biologiche presso l'Università di Napoli Federico II, nel 1977 con il massimo dei voti e la lode. (Tesi di Laurea Sperimentale in Chimica Biologica, Prof.ssa Benedetta Farina)

Coniugata con due figli Andrea ed Alessandro. Sesso F

Madrelingua: Italiana

Conoscenza lingua inglese scritta e parlata

Conoscenza informatica: Buon Livello

Residenza: viale Nicola Fornelli n.8, 80132 Napoli, Italia.

Istituto di Chimica Biomolecolare CNR via Campi Flegrei 34, 80078 Pozzuoli (Na) Italia

Tel +39 081 8675245; Fax +39 081 8041770; e-mail: Questo indirizzo email è protetto dagli spambots. E' necessario abilitare JavaScript per vederlo.;

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Dipartimento Scienze Chimiche e Tecnologie dei Materiali DSCTM 

Settore di competenza: Chimica delle Fermentazioni, Agroalimentare, Scienze della Vita


SCIENTIFIC ACTIVITIES


Interests

The research activity of Dr Nicolaus at CNR-ICB is mostly concentrated on:

a) Microbiology: Isolation of microbes from special environments using appropriate enrichment techniques, Purification and biochemical characterization of microbial cultures, Maintenance and upkeep of different stock cultures for varied laboratory usage, Growth kinetics of different organisms, optimization metabolites Production and evaluation of anti microbial activity of chemical agents and herbs. Strain identification by biochemical and molecular tests. Based on my research, I have good knowledge and experiences in fermentation and optimization process, strain identification, purification methods. Biochemistry: Protein and enzyme assays, Protein purification using Size exclusion, Ion exchange and Affinity chromatographic techniques, Analysis of proteins and enzymes using Native and SDS-PAGE. Production and purification of amylase from extremophiles: optimization of enzyme

production; The purification of enzyme was the next step by chromatographic and protein precipitation methods; Isolation and Kinetic characterization of microbial proteins (enzymes), bio-process scale-up by liquid, solid state fermentations, down stream processes and immobilization of microbial products. Isolation and purification of genomic DNA, restriction digestion. Chemistry: GC-MS, NMR, Spectroscopy Analyses, Natural ProductsBioinformatics: Blast analysis of Protein and Nucleotide sequences, Multi sequence, Alignment and ORF study by EMBOSS program. Molecular Biology: Isolation and purification of genomic DNA,

b) Biomolecules from agricultural waste such as polysaccharides, protein, lipids, vitamins, antioxidative compounds: isolation, optimization, purification, antioxidative assays, anticitotoxyc assays, chemical identification Biothecnological applications: cosmetics, food integrator, pharmaceutical, biodegradable plastic. The polysaccharides from natural sources have remarkable interest among several biotechnological products and they show a large range of applications in industrial field due to their commercial purposes

Some of them, for example, showing strong anti-genic and pathogenic activities, are employed successfully by the pharmaceutical industry for the formulation of vaccines, others are utilised as industrial food additivies taking advantage of their chemical-physical properties such as emulsifying, viscoelasticity, polyelectrolyte, adherence, bio-compatible, stabilizer, etc. One of the main problems of food industry is the management of waste and their conversion in products of higher value. Modern technologies eco-compatible, promote the use of food waste to obtain biopolymers that can be re-used in the same sector of the raw materials.

Major publications

  1. Spano' A, Cugliandolo C, Lentini V, Maugeri TL, Anzelmo G, Poli A, Nicolaus B. A novel EPS-producing strain of Bacillus licheniformis isolated from a shallow vent off Panarea Island (Italy). DOI: 10.1007/s00284-0130327-4 Current Microbiology: Volume 67, Issue 1 (2013), Page 21-29
  2. 148) Lama L., Poli A., Nicolaus B. Geobacillus thermantarcticus as source of biotechnological hermozymes and exopolysaccharides: a review. Current Trends in Microbiology (2013) vol 8 pp 1-12.
  3. Sadin Özdemir, Poli AnnaRita, Nicolaus Barbara- Biosorption of heavy metals (Cd2+, Cu2+, Co2+ and Mn2+) by thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus: equilibrium and kinetic studies Bioremediation Journal (2013) 17(2), 86-96.
  4. Nadja Radchenkova, Spasen Vassilev, Ivan Panchev, Gianluca Anzelmo, Iva Tomova, Barbara Nicolaus, Margarita Kuncheva, Kaloyan Petrov, Margarita Kambourova. Production and Properties of Two Novel Exopolysaccharides Synthesized by a Thermophilic Bacterium Aeribacillus pallidus 418. Appl Biochem Biotechnol. 2013 Sep;171(1):31-43. doi: 10.1007/s12010-013-0348-2. Epub 2013 Jun 30
  5. Yasar Yildiz S, Anzelmo G, Ozer T, Radchenkova N, Genc S, Di Donato P, Nicolaus B, Toksoy Oner E, Kambourova M.Brevibacillus themoruber: A Promising Microbial Cell Factory for EPS Production. J Appl Microbiol. 2013 Oct 7. doi: 10.1111/jam.12362
  6. Giuseppina Tommonaro, Angelamaria Caporale, Laura De Martino, Ada Popolo,Rocco De Prisco, Barbara Nicolaus, Gennaro Roberto Abbamondi and Carmela Saturnino Antioxidant and cytotoxic activities investigation of tomato seed extract. Natural Product Research 2014, doi.org/10.1080/14786419.2013.879474 
  7. Di Donato P., Finore I., Anzelmo G., Lama L., Nicolaus B., Poli A., 2014, Biomass and biopolymer production using vegetable wastes as cheap substrates for extremophiles, Chemical Engineering Transactions, 38, 163-168 DOI: 10.3303/CET1438028.
  8. Taurisano V., Anzelmo G., Poli A., Nicolaus B., Di DonatoP. Re-use of agro-industrial waste: recovery of valuable compounds bu ecofriendly techniques. International Journal of Performing Engineering vol 10 n 4 (2014) pp 419-425
  9. Finore, Di Donato, Mastascusa, Nicolaus, Poli, Fermentation Technologies for the Optimization of Marine Microbial Exopolysaccharide Production Mar. Drugs 2014, 12, 3005-3024; doi:10.3390/md12053005.
  10. Lama, Tramice, Finore, Anzelmo, Calandrelli, Pagnotta, Tommonaro, Poli, Di Donato, Nicolaus, Fagnano, Mori, Impagliazzo, Trincone. Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax. AMB Express 2014, 4:55.
  11. Finore, Di Donato, Poli, Kirdar, Kasavi, Toksoy Oner, Nicolaus, Lama. Use of Agro Waste Biomas for alpha Amylase Production by Anoxybacillus amylolyticus. Purification and Properties. Microbial and Biochemical Technology 2014 6,(6) 320-326

 Keyword

extremophile biomass waste biopolymer enzyme fermentation


OTHER