DiDonato-01
Dippolito-01
Dippolito-02
FIGURE_18
Impianto Palomonte
Poli-01
Poli-02
Poli_1
Poli_2
Poli_3
Poli_4
Poli_5

BIO-FERMENTATIONS FOR NEW MOLECULES


Activities

Production of both conventional and non-conventional microbe biomasses in controlled fermentation systems; development and optimization of fermentation strategies; use of agricultural and food industry wastes as inexpensive and ecocompatible substrates for fermentation; isolation and chemical, biochemical and genetic characterization of extremophilic bacteria as potential source of enzymes, lipids, antibiotics, polysaccharides and polyhydroxyalkanoates.

 

Expertise

Taxonomic assignment of new microorganisms; biotechnological characterization of enzymes from extremophilic bacteria; production, purification and chemical characterization of polymers if microbe origin; scale-up of the production of bacterial biomasses; optimization of downstream process parameters for the purification of the produced biomolecules; use of enzymes from extremophilic bacteria in biocatalysis; biocatalysis approaches with glycosynthases.

 

Coordinator 2017

Annarita Poli

Components

BIOPROCESSES FOR SUSTAINABLE ENERGY PRODUCTION


Activities

Development, optimization and integrated application of bioprocesses for the production of bio-oils and “bio-hydrogen”; studies of the regulation of the metabolic pathways leading to the production of the energetic vectors; development of efficient catalytic reactions, with low impact on the environment, and understanding of their molecular bases; development of multi-catalysis “cascade” processes for new synthetic methods.

 

Expertise

Bio-organic chemistry, biochemistry and microorganism physiology, with expertise in the characterization of new molecules by means of LC-MS, GC-MS, NMR and other spectroscopic techniques. Identification of metabolic pathways through studies of biosynthesis in microorganisms in culture, and expression of enzymatic activities by means of western-blot, use of purified enzymes and bacterial and fungine extracts as biocatalysts.

 

Coordinator 2017

Giuliana D'Ippolito

Components

BIOMASSES FOR THE INDUSTRY


Activities

Exploitation of agricultural and food industry wastes (tomatoes, lemons, carrots, fennels) as renewable source of molecules with high added value (polysaccharides, polyphenols, carotenoids, etc.). Waste recycling as a culture medium for extremophilic bacteria to be used for the production of enzymes of biotechnological interest.

 

Expertise

Optimization of bacterial fermentation processes; production and biochemical characterization of enzymes. Optimization of the retrieval of molecules with high added value through procedures with low impact on the environment. Characterization by means of NMR, IR, UV and MS of molecules with high added value. Bioassays on human and animal cell models.

 

Coordinator 2017

Paola Di Donato

Components

Publications of the group components in the years 2016-2017

  1. Ilaria Finore, Annarita Poli, Paola Di Donato, Licia Lama, Antonio Trincone, Massimo Fagnano, Mauro Mori, Barbara Nicolaus, Annabella Tramice. Hemicellulose extract from Cynara cardunculus: a source of value-added biomolecules produced by xylanolytic thermozymes Green Chemistry, 2016, 18, 2460-2472, doi: 10.1039/C5GC02774H
  2. Sadin Özdemir, Ersin Kılınç, Veysi Okumuş, Annarita Poli,Ida Romano, Barbara Nicolaus 2016).Thermophilic Geobacillus galactosidasius sp. nov. loadedγ-Fe2O3 magnetic nanoparticle for the preconcentrations of Pb and Cd. Bioresource Technology, 201, 269-275.
  3. Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A. Nesterenkonia aurantiaca, sp. nov., an alkaliphilic actinobacterium isolated from Cape King (Antarctica). Int J Syst Evol Microbiol. 2016 Jan 26. doi: 10.1099/ijsem.0.000917.
  4. 'Ippolito G, Sardo A, Paris D, Vella FM, Adelfi MG, Botte P, Gallo C, Fontana A. Potential of lipid metabolism in marine diatoms for biofuel production. Biotechnol Biofuels. 2015 Feb 22;8:28.
  5. Pradhan N, Dipasquale L, d'Ippolito G, Panico A, Lens PN, Esposito G, Fontana A. Hydrogen Production by the Thermophilic Bacterium Thermotoga neapolitana. Int J Mol Sci. 2015 Jun 4;16(6):12578-600
  6. Elisabetta Alberico,⊥, Alastair J. J. Lennox,⊥, Lydia K. Vogt, Haijun Jiao, Wolfgang Baumann, Hans-Joachim Drexler, Martin Nielsen, Anke Spannenberg, Marek P. Checinski, Henrik Junge, and Matthias Beller*, Unravelling the Mechanism of Basic Aqueous Methanol Dehydrogenation Catalyzed by Ru−PNP Pincer Complexes J. Am. Chem. Soc. 2016, 138, 14890−14904
  7. Alessandra Colombo, Andrea Schievano, Stefano P. Trasatti, Raffaele Morrone, Nicola D'Antona, Pierangela Cristiani Signal trends of microbial fuel cells fed with different food-industry residues International Journal of Hydrogen Energy - in press http://dx.doi.org/10.1016/j.ijhydene.2016.09.069.
  8. Pradhan, N., Dipasquale, L., d'Ippolito, G., Fontana, A., Panico, A., Pirozzi, F., Lens, P.N.L. and Esposito, G. Model development and experimental validation of capnophilic lactic fermentation and hydrogen synthesis by Thermotoga neapolitana. Water Research 2016, 99, 225-234

Recent publications of interest to the group components

  1. A. Trincone “Dal vino di Noè... alla Chimica verde”, Scienze e Ricerche n. 38, 1° ottobre 2016, pp. 19-22
  2. A. Trincone “Come è profondo il mare...”, Scienze e Ricerche n. 24, Marzo 2016, pp. 47-52

|Back|